basic principles of blood transfusion ppt

Blood banking is the process that takes place in the lab to make sure that donated blood, or blood products, are safe before they are used in blood transfusions and other medical procedures. Laboratory resolution of parentage problems. Transfusion therapy Packed red blood cells (pRBCs) and fresh frozen plasma (FFP) are components generally provided for canine transfusions. Fluid therapy is indicated either when there is a loss of fluid to any part of these compartments or there is a risk of loss of fluid. An Introduction to the Principles of Morals and Legislation - . Generally, the legal and ethical principles that apply to transfusion medicine are no different from those applicable to any medical interaction or intervention. Determination of ABO/Rh blood group by test tube technique. Blood Transfusion - . RBC TransfusionsAdministration Dose Usual dose of 10 cc/kg infused over 2-4 hours Maximum dose 15-20 cc/kg can be given to hemodynamically stable patient Procedure May need Premedication (Tylenol and/or Benadryl) Filter useroutinely leukodepleted MonitoringVS q 15 minutes, clinical status Do NOT mix with medications FRESH FROZEN PLASMA Used in case of multiple coagulation, deficiencies such as liver failure, warfarin OD, DIC, massive transfusion in bleeding patients, TTP, antithrombin III deficiency, hereditary angioma. is the procedure of introducing the blood of a donor, or pre-donated, Blood Transfusion - . There may be a chance of risk of infection. annette j. schlueter, md phd department of, An Introduction to Naturopathic Medicine - . an understanding of: available blood products, Transfusion medicine - . lisa breer, rn,bsn,nremt-b. There are more than 300 human blood groups but only a minority cause clinically significant transfusion reactions. Clipping is a handy way to collect important slides you want to go back to later. There are more than 300 human blood groups but only a minority cause clinically significant transfusion reactions. Screening for current or past infections, including: Human T-lymphotropic viruses (HTLV) I and II, Irradiation to blood cells is performed to disable any T-lymphocytes present in the donated blood. definition. Prescribing decisions should be based on national guidelines as well as hospital-developed guidelines approved by the medical staff (Transfusion Committee). From Petz and Swishers Clinical Practice of Transfusion Medicine, 2nd ed., 1989. However, sometimes, a patient may want to donate blood a couple of weeks before undergoing surgery, so that his or her blood is available in case of a blood transfusion. cheryl pollock 13 november 03. objectives. One unit of whole blood, once it's separated, may be transfused to several patients, each with different needs. About 6.8 million volunteers areblood donors each year. includes chapters on blood transfusion reactions and hemolytic disease of the 1. Antigen Antibody RatioThe speed by which antigen and antibody bind, is dependent on number of antibody molecules in the medium and the antigen sites available on the cell. 1. Blood for Britain (WW II) Project to collect large amounts of blood in NYC hospitals. Title: PowerPoint Presentation Last modified by: admin Created Date: 1/1/1601 12:00:00 AM Document presentation format: On-screen Show Other titles - A free PowerPoint PPT presentation (displayed as an HTML5 slide show) on PowerShow.com - id: 72e93b-ZTU2N . 16671st DocumentedAnimal-to-Human Transfusion Jean Baptiste Denis infuses 15-year-old boy with lambs blood. Hemolysis The antigen and antibody reaction where complement is activated and leads to breakdown of red cells and release of hemoglobin is called hemolysis. 18181st DocumentedHuman-to-Human Transfusion Following a 150-year transfusion hiatus, James Blundell transfuses patient with blood from a human donor. introduction of new matching blood into the bloodstream or/and of any. We've updated our privacy policy. Masks are required inside all of our care facilities. Wound Care Made Incredibly Easy! blood may be need to be washed to remove the debris before re-infusion. Boasting an impressive range of designs, they will support your presentations with inspiring background photos or videos that support your themes, set the right mood, enhance your credibility and inspire your audiences. Blood Transfusion - . History - Karl Landsteiner Discovered the ABO Blood Group System in 1901 He and his five co-workers began mixing each others red cells and serum together and inadvertently performed the first forward and reverse ABO groupings. Principles Of Genetics Snustad Simmons 6th Edition is easy to get to in our digital library an online right of entry to it is set as public for that reason you can download it instantly. Principles of Transfusion Medicine. PowerPoint Presentation Simulations for Clinical Excellence in Nursing Services SCENS Welcome To Infusion: IV start and Blood Infusion: IV Start and Blood SCENS Learning Objectives Discuss the appropriate steps to initiate intravenous access Demonstrate the steps required to initiate blood infusion therapy Salah Al - Kubaisi, Medicine 5th year, 4th lecture (Dr. Sabir). For example, within the A blood group, there are subtypes such as A1 and A2, and within the B blood group, there are subtypes such as B1 and B3. Clipping is a handy way to collect important slides you want to go back to later. Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. 184 Comments Please sign inor registerto post comments. 2. Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. 1800sAll Manner of Blood Collection Devices Utilized (You think present-day donor centers sometimes face challenges in recruiting repeat blood donors?) Principles. Complete Webcast library As a member, you will be able to access all the content. chapter summaries, and case studies; chapter PowerPoint slides; test bank; and image bank. health care service. The number of blood units donated is about 13.6 million a year. BLOOD TRANSFUSION - . Albumin, immune globulins, and clotting factor concentrates may also be separated and processed for transfusions. Historical Overview In 1492, first blood transfusion involving Pope Innocent VIII. mohammed a. omair, mbbs, sf rheum organizer 341 med consultant, Progress and Future of Transfusion Medicine - . Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. INTRAVENOUSLY. Transfusion Medicine - . Blood grouping is a crucial aspect of modern medicine that allows us to identify and classify the various types of blood that exist within the human body. BLOOD TRANSFUSIONS INVOLVES THE USE OF WHOLE BLOOD , Blood Transfusion - Prepared by : shurouq qadous & samah ishtieh. Blood has many different functions, including: transporting oxygen and nutrients to the lungs and tissues forming blood clots to prevent excess blood loss carrying cells and antibodies that fight infection 1940s Making Plasma Products Edwin Cohn develops the first fractionation technique to break down the components of plasma into Cohn fractions. John Elliott develops a vacuum bottle/blood container. Eg: Parent to child, TYPES OF BLOOD COMPONENTS RED BLOOD CELLS WHOLE BLOOD PLATELETS FRESH FROZEN PLASMA ALBUMIN CRYOPRECIPITATES, ABO BLOOD GROUP DONORS UNIVERSAL DONOR UNIVERSAL ACCEPTOR. A blood transfusion provides blood or blood components if you've lost blood due to an injury, during surgery or have certain medical conditions that affect blood or its components. Blood transfusion and its moral implications, Indications and complications of blood transfusion. Nursing Tutor Fetal Rh typing from amniocenthesis, and treatment with small dose of Rh immune serum will prevent sensitization during pregnancy. The portion of the plasma that contains clotting factors that help to control bleeding. Looks like youve clipped this slide to already. PRBC infused when acute blood loss, profound anemia with, Units of blood stored in the blood bank fridge or, Injection Frusemide (Lasix) 40mg (IV or PO) often given with, Dont put the blood bag in the microwave oven or in hot, If any adverse reaction occurs dont discard the blood. module 1. what is laboratory medicine?. topic modules. We've encountered a problem, please try again. But, the importance of ABO supersedes all From Petz and Swishers Clinical Practice of Transfusion Medicine, 2nd ed., 1989. blood components namely packed red blood cell (PRBC) concentrate, platelet concentrate, fresh. DIRECTED:- Blood products donated by person for transfusion to specified recipient. Anti-Rh antibodies are not spontaneously formed in Rh individuals. Place the original record in patient file. Blood Transfusion: Procedure and Complications, Blood Transfusion (Principles and procedure), Blood transfusion by M.Sc.Second year, 2020-21 Btach, SVBCON, Silvassa, Safe blood transfusion practices and policy of hospital, Blood components tranfusion guidelines update, Blood transfusion -procedure,precaution and complication, Blood product transfusion and massive transfusion. Rate 14-20 20-30 30-40 >35 Urine . (N) Nursing Eductor Al-Ahsa Hospital Kingdom of Saudi Arabia. Blood Erythrocytes (red blood cells) Leukocytes (white blood cells) Platelets C L GUPTA EYE INSTITUTE MORADABAD UTTER PRADESH, Investigations in hemorrhegic disorders ppt Prashant Mune, Blood coagulation disorders and laboratory diagnosis, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. Use of blood products also puts patients with haemophilia at risk of additional complications, such as allergic and transfusion reactions, and fluid overload. Activate your 30 day free trialto continue reading. Before a transfusion can take place, the donor's blood is tested against the recipient's serum, or plasma without the clotting proteins, to see if there is a reaction. Volunteer blood donors must pass certain criteria, including the following: Must be at least 16 years of age, or in accordance with state law, Must pass the physical and health history exam given before donation. University Wide Seminar By Natnael Dechasa at Dire Dawa University regarding vital statistics related to maternal health in indIA.pptx, 1- Introduction to Therapeutic Exercises.pptx, acls-advanced-cardiac-life-support-certification-course-certificate (1) (1).pdf, Calculation of medication in Pediatric., walaa.ppt, formulation and evaluation of cosmetics.pptx, Hospital Quality Accreditation - HA202 - 2023, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. Nursing 1st year. Verify that there is a signed Consent and Blood Transfusion form with the patients name and medical record number documented on it. WR(JP|!%)RL\*QhC(-iLw`! 1. FACTORS AND PLATELETS. Tap here to review the details. Body recovers the Blood very quickly: Blood plasma volume- within 24 - 48 hours. It is not used if the patient is hypovolemic. The SlideShare family just got bigger. It appears that you have an ad-blocker running. The SlideShare family just got bigger. Good luck! All the units and administration sets must be returned to blood bank. From Petz and Swishers Clinical Practice of Transfusion Medicine, 2nd ed., 1989. of antibody, while some (e.g., intravenous immune globulin and plasma products) contain a large amount. By accepting, you agree to the updated privacy policy. The SlideShare family just got bigger. 4. Click here to review the details. Criterion Three Managing blood and blood product safety Blood must be stored and handled appropriately to prevent risk to patients Wastage of blood should be avoided Systems around receipt, storage, collection, and transport must address these risks Issue - outsourced blood provision services Storage and handling 7.7 Blood and blood products . College of How We Make Blood Components Collection Process (1) Via Whole Blood Donation: Whole blood is collected from healthy blood donors into sterile blood bags that contain anticoagulant-preservative. Transfusion of blood and its components. blood blank practices indication to blood transfusion complication alternative, BLOOD TRANSFUSION - Blood transfusion. Blood banks and healthcare providers ensure transfusions are a safe, low risk treatment. Each unit of blood is broken down into components, such as red blood cells, plasma, cryoprecipitated AHF,and platelets. understand, Transfusion Medicine: Transfusion Reactions and Massive Transfusion - . Free access to premium services like Tuneln, Mubi and more. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. This is an automatically translated article. (N) Nursing Eductor Al-Ahsa Hospital Kingdom of Saudi Arabia. In 1902, his proteges identify a group AB individual for the first time. Technical Manual of the American Association of Blood Banks. Cryoprecipitate Platelete Blood Components Preparation Based on different specific gravities RBC : 1.08-1.09 Platelet : 1.03-1.04 By using differential centrifugation,blood components separated into layers From a unit of whole blood,the centrifuged product settle out into RBC,WBC & platelet-rich plasma(PRP). GENERAL PRINCIPLES OF BLOOD TRANSFUSION - 1963 - Transfusion - Wiley Online Library GENERAL PRINCIPLES OF BLOOD TRANSFUSION First published: May 1963 https://doi.org/10.1111/j.1537-2995.1963.tb04684.x Citations: 10 PDF Tools Share Get access to the full version of this article. IgM react at room temperature 20-25C and it does not cross the placental barrier. Tap here to review the details. alternatives to transfusion for surgical patients. From Transfusion, Vol. Prepare donor and recipient's blood sample: Donor's red cells and recipient's serum/plasma. Red Blood Cells - in about 3 weeks. Principles of Transfusion Medicine Objectives At the conclusion of this presentation, participants will be able to summarize, from an evidence-based perspective, The means by which blood components are manufactured, The approved uses of RBCs, platelets, plasma & cryoprecipitate, The standard of care for the use of CMV-seronegative and irradiated cellular blood products. Sana Usmani The blood groups are classified based on the presence or absence of a particular antigen. In 1665, an English physiologist, Richard Lower, successfully performed the first animal-to-animal blood transfusion that kept ex-sanguinated dogs alive by transfusion of blood from other dogs. utilitarianism. From Zmijewskis Immunohematology. 2.2: Blood group antibodies. frozen plasma and cryoprecipitate. The basic principles of blood safety are enough safe blood donors, a responsible blood transfusion service which can ensure appropriate and safe processing and testing of blood, and appropriate use of blood. Prepare 3-5% saline cell suspension of red cells. Once produced they persist for years and can produce serious transfusion reaction during 2nd transfusion. Facts about blood banking According to the American Association of Blood Banks as of 2013: About 36,000 units of blood are needed every day. Maintain intravenous (IV) access (do not flush the existing line and use a new IV line if required). Blood Banking and Transfusion Medicine Basic Principles and Practice 2nd Edition - October 18, 2006 Write a review Authors: Christopher Hillyer, Leslie Silberstein, Paul Ness, Kenneth Anderson, John Roback eBook ISBN: 9780702036255 Hardcover ISBN: 9780443069819 Purchase options Select country/region Info/Buy Institutional Subscription DURING THE PROCEDURE:- Note for the reactions. Blood Basics Blood is a specialized body fluid. risks. We've encountered a problem, please try again. case scenario, TRANSFUSION MEDICINE - . 2. The information on the www.vinmec.com is ONLY for references. You can read the details below. TRANSFUSIONS USUALLY INVOLVE THE USE OF TWO SOURCES OF BLOOD TRANSFUSION PERMIT Signed blood transfusion permit on chart Acute patients must get a permit for each admission LTC residents-permits must be within the last 30 days 3 Verify Physicians order in HMS. 3. Vinmec is happy to send you the latest news by email. FFP administered through standard blood administration set. 1900ABH Blood Group System IDd Karl Landsteiner discovers ABH system when he types individuals as (what we now call) group A, group B, and group O. In 1666, Richard Lower English physician reported the first successful transfusion between animals. The SlideShare family just got bigger. Patients should be under regular visual observation and, for every unit transfused, minimum monitoring should include: Pre-transfusion pulse (P), blood pressure (BP), temperature (T) and respiratory rate (RR). ISBT Education Portal | The International Society of Blood Transfusion (ISBT) Homepage Resources ISBT Education Portal ISBT Education Portal Access congress webcasts, webinar recordings, e-learning modules and more. 2. Units of blood stored in the blood bank fridge or theatre fridge. Introduction Transfusion of blood and its products is very common medical procedure in the practice of modern medicine. There may be a chance of risk of infection 3. Do not sell or share my personal information, 1. central pennsylvania nant chapter spring conference april 15. Nursing, AIIMS. Add two drops of recipient's serum and one drop of donor cell suspension. AntigenAntigen is a substance which elicits immune response. basic understand of the laboratory staff blood screening procedures; . If the blood cells clump together in response to the anti-Rh serum, it indicates that the blood is Rh-positive. Factors influencing agglutinationThe following factors affect the process of agglutination. The two most important in clinical practice are the ABO and Rh systems. Clipping is a handy way to collect important slides you want to go back to later. Fact sheets -2. . Coverage begins with skin anatomy and physiology, wound progression and healing, and factors that can delay or complicate healing. It appears that you have an ad-blocker running. RED BLOOD CELLS, WHITE BLOOD CELLS, PLASMA, CLOTTING objectives. 1914Modern Anticoagulation is Born Citrate first used for blood anticoagulation purposes. Verify Hemoglobin and Hematocrit level (If there joan macleod, mlt, dba district technical manager blood transfusion, Transfusion Medicine: A History - . 5.4 Transportation of Blood and Blood Components 66 6 PREPARING BASIC SOLUTIONS 77 6.1 Copper Sulfate Solutions 78 6.2 Antiseptic Solutions 79 6.3 Saline Solutions 79 The Best Research of the Year Presented at the National Conference, prepared 1- Introduction to Therapeutic Exercises.pptx, Physicians Email List - Accurate List Inc, Calculation of medication in Pediatric., walaa.ppt, Scope of Social Work in the Mental Health sector.pptx, acls-advanced-cardiac-life-support-certification-course-certificate (1) (1).pdf, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. If any adverse reaction occurs dont discard the blood bag tubing and attached IV solution. Universal Donor; Suitable for all? The era of blood transfusion, however, really began when William Harvey described the circulation of blood in 1616. It reduces risk of transmission of diseases & potential transfusion complications. In contrast some of the antibodies contain exclusively kappa or lambda light chains are known as monoclonal antibodies. objectives. At Another Johns Hopkins Member Hospital: Masks are required inside all of our care facilities, COVID-19 testing locations on Maryland.gov, Pediatric Gastroenterology Hepatology and Nutrition. This H substance is present in unmodified form in individuals with blood type O. BLOOD SALVAGE:- It is an autologus donation that involves suctioning blood from body cavities, joint spaces, or other closed body sites. Most blood donors are volunteers. An Introduction to the Principles of Transfusion Medicine. Activate your 30 day free trialto continue reading. Blood transfusion involves two procedure that is - Collection of blood from donor And Administration of blood to the recipient. Blood can be optimally utilized by the use of specifically required components instead of whole blood RBC FFP PLT, Plasma Platelets Mononuclear Cells Granulocytes Red Blood Cells Whole Blood Whole Blood (vein) (vein) The Principles of Apheresis Anticoagulant added Remaining blood constituents returned Blood constituents separated by centrifuge and selectively collected, Plasma Collected Via Apheresis Typically200 mLto800 mL FFP Made from Apheresis, Donor sample tubes being readied for testing, Infectious Disease Testing (Abbott PRISM), Platelet Bacterial Detection QC Testing by BacT/ALERT, Testing ABO Rh RBC Antibody Screen Infectious Diseases Syphilis HBsAg Anti-HIV-1/2 Anti-HBc, 2023 SlideServe | Powered By DigitalOfficePro, An Introduction to the Principles of Transfusion Medicine, - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -. Before transfusion If the blood bracelet doesnt match with patient ID band or if the blood bracelet doesnt present DONT INFUSE IT. By accepting, you agree to the updated privacy policy. introduction It is the administration of packed red blood products separated in the blood bank from collected whole blood. 3. risks & benefits. The science of Immunohematology deals with the basic principles of antigen and antibody structure, to understand the principles of compatibility testing and transfusion reactions the basic knowledge of Immunohematology is essential. Thank you for subscribing. Institutional Login Learn faster and smarter from top experts, Download to take your learnings offline and on the go. Introduction to the Principles of Laboratory Medicine - . Appointments & Access Contact Us dr. ayesha junaid mbbs,mcps,fcps. The Collection Process Phlebotomy (16 Gauge Needle) Arm Disinfection Prior to Donation, The Collection Process Multi-bag whole blood collection kits Flow of whole blood into primary bag, Centrifuge (low g forces) WHOLE BLOOD Blood Component Manufacture from Whole Blood (As Done in the USA) RBCs + Platelet-Rich Plasma, Centrifuge Used for Component Manufacture, Centrifuge (higher g forces) Platelets + or Plasma Freeze Blood Component Manufacture from Whole Blood Leukoreduce Possibly irradiate Other RBCs + Platelet-Rich Plasma Fresh Frozen Plasma (FFP) or Plasma Frozen with 24 hours, Thaw (4 C) Centrifuge Blood Component Manufacture from Whole Blood Fresh Frozen Plasma (FFP) Cryoprecipitate + Cryo-Reduced Plasma. The blood groups were divided in A, B and O. Anti-i : this is a cold reactive auto-agglutinin. Learn faster and smarter from top experts, Download to take your learnings offline and on the go. 1.6 Introductory Module: Guidelines and Principles for Safe Blood Transfusion Practice 11 1.7 Module Objectives 12 1.8 Planning Your Study 14 . $no4mo}={Mj|>KO4&9}d(uj#kw~nZ%9Gyrx2_\7n\|Tp2N_vr?yx6diOL `Z L_cFp;Ld%=2{e KO~G/T|MG/>{j`kYKL `Z tk8K8K8k8k8 gLL^h\lq5Z5Zswj\Vj=2*D. Principles of Transfusion Medicine Outline Brief Historical Perspective The Manufacture of Blood Components Evidence-Based Transfusion Indications for: (Whole Blood) RBCs Plasma (including use for major trauma) Platelets Cryoprecipitate Special (CMV-negative and/or Irradiated) Products, Principles of Transfusion Medicine A Brief History. several centuries. Senior Medical Officer at hindu mission hospital, Do not sell or share my personal information. Start Blood Transfusion within 30 minutes of blood being removed from the refrigerator. This Anti-H of Oh is clinically significant, warm antibody reactive at 37C. Plasma serves many functions, including the following: Balances the levels of sodium and potassium. We've updated our privacy policy. Dried plasma package developed for ease of transport, packaging and storage. T0%ULbeitaY3B0,+8Je+ #WN`#\agV3LD .3 B;R&HLwaBw orracha kok - kaew , md. "General Principles of Blood Transfusion" lends needed reinforcement to the Forgotten Front of American medicine. what is blood made up of?. Platelets. C nhiu quan im cho rng ngi c nhm mu A Nhm mu l mt nhm cc t bo hng cu v c xc nh cn c da vo c tnh mi loi khng nguyn khc nhau. 2008 my lecture introduction to hematology, Introduction to hematology, Al-YAQIN DIAGNOSTIC ULTRASONIC CLINIC BAGHDAD, Lecture 1: Hematology introducion For TID and HIV Medicine MSc students, Introduction to veterinary hematology / Assist.Prof.Sr. The watery, liquid part of the blood in which the red blood cells, white blood cells, and platelets are suspended. assistant professor dr. talib hussein kamoona. Supplies nutrients to tissues: O2, hormones, glucose 2. Annually, more than21 million blood components are transfused. medicines and healthcare products regulatory agency (mhra) eu. blood bank. Blood transfusion guidelines in clinical practice, Blood transfusion and its reactions in obstetrics, Blood transfusion, Nutrition and water & electrolyte balance, Blood products and massive blood transfusion, Blood transfusion & Blood Component Therapy-An Update, Blood Transfusion Center by Slidesgo.pptx, Blood transfusion and transfusion reactions, Blood Transfusion (a nursing procedure) by www.nursesinfosite.blogspot.com, Blood transfusion reactions and complications, Indications and complications of blood transfusion, Blood transfusion in oral and maxillofacial surgery, Blood, Blood transfusion and Blood products, Jehowah's witnesses and blood conservation strategies by Dr.Minnu M. Panditrao, Transfusion support in neonates and paediatric patients [autosaved], Blood transfusion -procedure,precaution and complication. All blood products must be infused through proper administration set. In this report, "transfusion" refers to any situation in which a patient receives any component of human blood from a single or multiple donors. Some blood products (e.g., washed or . Activate your 30 day free trialto unlock unlimited reading. professor of pathology consultant haematology incharge blood, TRANSFUSION MEDICINE - . Dont exceed beyond 300mm of Hg otherwise it may cause HEMOLYSIS, 2023 SlideServe | Powered By DigitalOfficePro, - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -. It reduces risk of transmission of diseases & potential transfusion complications. 1unit can safely infuse over 1-2 hours. This treatment also provides blood if your body is not making blood properly on its own. 2.3: Testing for red cell antigens and antibodies in the laboratory. kathleen m. madden md jay s. raval md department of pathology. Increasing advancement in. It appears that you have an ad-blocker running. We've encountered a problem, please try again. Get powerful tools for managing your contents. 1939/40Rh and Cause of HDFN Discovered Levine, Wiener, and colleagues combined their efforts in making these seminal discoveries From Netter Monograph Series. We've updated our privacy policy. ngi, cc nhm mu ngi c chia lm nhiu loi v mi loi c c trng ring.

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basic principles of blood transfusion ppt